The supercritical fluid used for analysis The mobile phase in a chromatograph is a supercritical fluid (like CO2) with good mass transfer and dissolving properties. The system temperature and pressure, as well as the mobile phase’s composition and flow rate, are adjusted to optimize the preparation and analysis conditions.
Technical Features of Analytical Supercritical Fluid Chromatography
01.
Quicker Elution
Elution greatly cuts down on analysis time and improves preparative efficiency because it is usually carried out at flow rates three to five times higher than traditional HPLC.
02.
The Eco-Friendly Mobility Stage
The use of supercritical CO₂ combined with a minimal amount of organic modifier as the mobile phase offers a greener, more sustainable approach for both analytical and preparative separations.
03.
Compatibility with Hydrophilic Macromolecules
For some hydrophilic biomolecules, the method works effectively because it maintains both their biological activity and structural integrity during the separation process.
04.
complimentary Retention Behavior
SFC offers complimentary capabilities that improve overall separation techniques, and it displays retention characteristics that are different from those of GC and HPLC.
Supercritical Fluid Chromatography System
Model
SFC-lab 20
SFC-lab 150
CO2 pump flow rate
≤20mL/min
≤150mL/min
Entrainer pump flow rate
≤10mL/min
≤100mL/min
Pressure range
≤25MPa
≤25MPa
Column oven temperature
Room temperature~70℃
/
Wavelength range
190-700nm
Light source
Deuterium lamp and tungsten lamp
Back pressure range
0~25MPa
0~20MPa
Adapt to the specifications of
chromatographic columns
Analytical SFC Chromatography (Supercritical Fluid Chromatography) utilizes supercritical CO₂ as the mobile phase, resulting in quick, efficient, and environmentally friendly separation for analytical and preparative operations.
Preparative SFC concentrates on large-scale purification, whereas analytical SFC is utilized for exact analysis. Both utilize supercritical fluids to improve separation efficiency and sustainability.
SCF Chromatography is a cost-effective and eco-friendly alternative to typical liquid chromatography systems. It uses supercritical CO₂ with modest amounts of organic modifiers to reduce solvent waste.
An SFC System allows for faster elution, flexible flow rates, and more control over temperature and pressure, resulting in faster, cleaner, and more accurate separation.
SFC Purification is ideal for hydrophilic macromolecules as it preserves biological activity and structural integrity, ensuring reliable and high-quality analytical outcomes.
Contact Intech Analytical Instruments
Office Address
Plot no, 680, Rd Number 15, Vasanth Nagar, Kukatpally, Hyderabad, Telangana 500085