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Analytical Supercritical Fluid Chromatography

Analytical Supercritical Fluid Chromatography

The supercritical fluid used for analysis The mobile phase in a chromatograph is a supercritical fluid (like CO2) with good mass transfer and dissolving properties. The system temperature and pressure, as well as the mobile phase’s composition and flow rate, are adjusted to optimize the preparation and analysis conditions.

Technical Features of Analytical Supercritical Fluid Chromatography

01.

Quicker Elution

Elution greatly cuts down on analysis time and improves preparative efficiency because it is usually carried out at flow rates three to five times higher than traditional HPLC.

02.

The Eco-Friendly Mobility Stage

The use of supercritical CO₂ combined with a minimal amount of organic modifier as the mobile phase offers a greener, more sustainable approach for both analytical and preparative separations.

03.

Compatibility with Hydrophilic Macromolecules

For some hydrophilic biomolecules, the method works effectively because it maintains both their biological activity and structural integrity during the separation process.

04.

complimentary Retention Behavior

SFC offers complimentary capabilities that improve overall separation techniques, and it displays retention characteristics that are different from those of GC and HPLC.

Supercritical Fluid Chromatography System

Model

SFC-lab 20

SFC-lab 150

CO2 pump flow rate

≤20mL/min

≤150mL/min

Entrainer pump flow rate

≤10mL/min

≤100mL/min

Pressure range

≤25MPa

≤25MPa

Column oven temperature

Room temperature~70℃

/

Wavelength range

190-700nm

Light source

Deuterium lamp and tungsten lamp

Back pressure range

0~25MPa

0~20MPa

Adapt to the specifications of chromatographic columns

4.6mm or 10mm

20 or 30mm

Data processing unit

Greenchrom Chromatographic workstation

Related Products

SFC-Dynamic Axial Compression Column

SFC-Dynamic Axial Compression Column

CO₂ Booster Station

CO₂ Booster Station

Frequently Asked Questions

Analytical SFC Chromatography (Supercritical Fluid Chromatography) utilizes supercritical CO₂ as the mobile phase, resulting in quick, efficient, and environmentally friendly separation for analytical and preparative operations.

Contact Intech Analytical Instruments

Office Address

Plot no, 680, Rd Number 15, Vasanth Nagar, Kukatpally, Hyderabad, Telangana 500085

Lab Address

Plot No: 13, Sy No: 62/1/A,
Kakateeya Nagar, Bahadurpally, Dundigal,
Gandimaisamma, Medchal - 500043

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